Hepatocytes from embryonic stem cells: Prometheus revisited?
نویسندگان
چکیده
منابع مشابه
Regenerative medicine and stem cells: prometheus revisited.
to damaged tissues and organs. In other words, regenerative medicine is the ‘process of replacing or regenerating human cells, tissues or organs to restore or establish normal function’ [4, 5] . It includes a group of biomedical approaches to clinical therapies that may involve the use of stem cells. Examples include: the injection of stem cells or progenitor cells (cell therapies), the inducti...
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Differentiation of human embryonic stem cells (hESCs) to specific functional cell types can be achieved using methods that mimic in vivo embryonic developmental programs. Current protocols for generating hepatocytes from hESCs are hampered by inefficient differentiation procedures that lead to low yields and large cellular heterogeneity. We report here a robust and highly efficient process for ...
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The aim of this study was to isolate mouse embryonic stem cells from late blastocyst stage embryos and to use them as a model system for the study of hematopoietic induction outside the embryo by coculturing of embryonic stem cells with bone marrow stromal cells. Blastocyst stage embryos from pregnant NMRI mice were obtained and cultured for 1-2 days in DMEM medium. The inner cell masses formed...
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Purpose: Differentiation of Mouse embryonicstem cells into Hematopoietic cells. Materials and Methods: In this study, we used EB formation system for Hematopoietic differentiation of mouse embryonic stem cell (Royan B1) in suspension culture. EBs cultured in medium with Hematopoietic inducer cytokines (SCF, TPO, GMCSF, IL3, Flt3 and EPO) .presence of hematopoietic differentiated cell assessed ...
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ژورنال
عنوان ژورنال: Hepatology
سال: 2007
ISSN: 0270-9139,1527-3350
DOI: 10.1002/hep.21574